Microscopic Analysis

Besides research, microscopic analysis is also applied for quality control and defect analysis. It shows the correlation between structures of polymers and their mechanical properties. In addition, microscopic analysis is important for understanding the influences of processing parameters on materials and their mechanical failures.

Thin sections of thermoplastic polymers are prepared by microtome for some microscopic analysis using transmitted light. For very soft materials, the cooling of specimens is important. Samples can be embedded in acrylic resin for long-term storage the specimens. Reinforced polymers can be first embedded in epoxy resin and then be polished. Samples for SEM will be sputter coated with a gold, platinum or chromium layer to avoid charging.

Depending on different illumination systems, there are transmitted-light and reflected-light microscopes.

  • In transmitted light microscopy, the light passes through the section and then be captured by the objective. Therefore, transparent thin sections are needed.
  • In reflected-light microscopy, illuminating light reaches the specimen, which may absorb some of the light and reflect some of the light, either in a specular or diffuse manner. Light that is returned upward can be captured by the objective in accordance with the objective’s numerical aperture.
Transmitted lightBright fieldImpurities
PolarizationSpherulite structure, orientations
Phase contrastPolymer blends
Reflected lightBright / Dark fieldImpurities, cracks
Differential interference contrastFiber distribution
FluorescenceCracks
  • Due to its large depth of field, the scanning electron microscope is preferred for imaging unevenly structured surfaces.
  • For the material identification of microscopic samples, energy dispersive X-ray microanalysis (EDX) is used in the SEM to detect elements and their distribution.
  • Industrial computed tomography (CT) is used to analyze the internal structure of components without contact and without causing damage. Classic applications include porosity and void analysis, target/actual comparison (deviations in the shape of the model and component), and non-destructive wall thickness analysis.

For sample preparation

DeviceDevice dataAim
Grinding/polishing equipmentWater-cooledPreparation of ground sections; Surface treatment
Sled microtomes, rotary microtomesCutting thickness: material-dependent (min. 3 µm); Freezing deviceThin section preparation; Surface treatment
Precision cutting machinesCutting discs: 75-203 mm; Water-cooledFast and precise cutting of workpieces
Saw microtomesCutting thickness: 100 µm to 1 cm;
Sample size: 25 mm diameter;
30 mm height
Production of plane-parallel cuts of samples
Plasma etching systemMicrowave-generated plasma;
Chamber volume: 43 liters;
Gas: O₂, He, Ar
Physical etching
Sputter coater systemLayer thickness: 0.5–2 nm;
Target materials: Au, Pt, Pd, C
Metallic, conductive coating of the sample surface for SEM examinations

For microscopic analysis

DeviceDevice dataAim
Light microscopes
(reflected light microscopy)
Magnification: 25 to 1000x;
Bright field, dark field, and polarization contrast;
DIC and C-DIC contrast;
Fluorescent lamp;
Digital image capture
Examination of ground sections;
Optical representation of: fillers and reinforcing materials, defects, and material deformations
Light microscopes
(transmitted light microscopy)
Magnification: 25 to 1000x;
Bright field, polarization, and DIC contrast;
Phase contrast;
Tilt compensator B (5);
Digital image capture;
Heating table device
Examination of thin sections;
Optical representation of: material structure, defects, and particles
Stereomicroscope
(transmitted light and reflected light microscopy)
Magnification: 5 to 150x;
Transmitted light: Bright field and polarized contrast;
Oblique light;
Reflected light: Bright field contrast, oblique light
Surface analysis;
Spatial observation of objects
Large-area optical stress testerField of view: 300 mm; Linear and circularly polarized lightDetermination of residual stresses and orientations
Canning electron microscopeMagnification: 20 to 150,000x;
secondary electron and backscattered electron detector;
digital image capture;
EDX system
Structure and fracture surface analysis;
Element analysis
Computed tomography system (Sub-µCT)Detail recognition: from 500 nm;
Max. object size: 65 mm diameter;
Max. voltage: 160 kV
Non-destructive and three-dimensional examination of components

The Institute of Polymer Technology organizes seminars with practical demonstrations and symposias on selected topics regularly. An overwiew of the currently planned events can be found on here.


Contact person

Dipl.-Ing. (FH) Gabriela Riedel

Head of Testing Laboratories

Institute of Polymer Technology